Altogex™

Umbilical Cord blood Native Nuecleated cells (UCB-NNC)

Human cord blood native nucleated cells (HCBNNCs) are separated from umbilical cord blood using a proprietary protocol. HCBNNCs are screened for the expression of immune marker CD45 and their viability is assessed using Acridine Orange or DAPI staining and our state of the art fully automated cell counting machine. This highly purified population of cells makes it possible to further isolate specific and more rare populations, as well as additional downstream processes.

The Altogex™ fraction is a result of a validated technology composed of lymphocytes (T cells, B cells, and natural killer cells), monocytes, dendritic cells, and stem/progenitor cells. These cells are important cellular components of the immune system and essential in effective humoral and cell-mediated immune responses. These cells are commonly used in basic science research as well as IND (Investigational New Drug)-approved clinical applications in the fields of immunology and infectious disease. The long-term goals of many of our customers include vaccine development and cellular transplantation.

Description

Human cord blood includes many types of immune cells and is considered a rich source of hematopoietic stem and progenitor cells. The collection of cord blood is a non-invasive and painless procedure performed at one of our partnering recovery agencies by trained procurement staff. Maternal blood for the fresh whole cord blood is obtained and must test negative for HIV, HBV, HCV, CMV, Syphilis, WNV, HTLV I/II Ab, Chagas, and Lyme Disease. The blood is then transferred to our cell manufacturing facility where specific primary cells are processed, analyzed, and vialed. Cellular characteristics, such as phenotype, viability, purity, and cell count, are checked to meet rigorous federal standards to ensure high-quality cells have been obtained.

Cells are obtained using approved consent forms and protocols. They are assessed using federal guidelines.

Hematopoietic stem cells are adult stem cells that are capable of differentiating into all blood cell types. This process ensures blood homeostasis of blood throughout adult life. The formation of blood cells first occurs during embryonic development in blood islands. These are located within the yolk sack and in the aorta-gonad-mesonephros region. HSCs then migrate in later stages of embryonic development to both the placenta and fetal liver. These sites are the main compartments of blood cell formation before birth. Shortly before birth, HSCs migrate to the bone marrow. It is here that blood cell formation occurs postnatally.

HSCs were discovered in the 1960s. Identifying these somatic cells has prompted worldwide interest in stem cell research and prompted researchers to consider stem cell niches, cellular self-renewal, and the conserved mechanism of asymmetric cell division. The HSC model is used to better understand blood cell formation or hematopoiesis and to determine mechanisms underlying cell renewal and differentiation. HSCs or cells derived from HSCs have been used in vivo to treat a wide range of disorders and diseases related to the blood. These include leukemia, sickle cell disease, and severe combined immune deficiency (SCID). Many sources hold HSCs, including the blood of the umbilical cord, bone marrow, peripheral blood, or differentiated ES (embryonic stem cells) and iPS (induced pluripotent stem cells). The number of successfully isolated HSCs is dependent on the source used.

Phenotyping analysis of native HSCs by flow cytometry and hematology analysis

Altogex™ is routinely analyzed by hematology and flow cytometry based on various extracellular and intracellular markers.

Upon request, Leo Corps and affiliative companies also offer researchers a wide range of monoclonal antibodies ideal for the characterization of HSCs and HSC-derived cells, including CD34, CD38, CD133, CD117, and  Sca-1. Our fluorescent antibodies are useful in characterizing cell populations using flow cytometry using established protocols and antibody panels.

For details about our specific flow cytometry antibodies or hematology reagents for cell analysis, please contact us.

Weight 1 lbs
Dimensions N/A
Size

1cc ( 6 Million), 2cc ( 12 Million ), 3cc and 5cc (30 Million)
Manufacturer

Altogex Biotech
U/M

1cc ( 6 Million), 2cc ( 12 Million ), 3cc and 5cc  (30 Million)
Storage

Sterile Vial
Shipping Temp/Condition

Frozen ( -81 celcius)
Storage Temp

(-80 celcius or lower)
Media Expiry, 4C

N/A
Media Expiry,    -20C

N/A
Preservative

DMSO (5%)
Unless otherwise specifically indicated, Leo Corps and its affiliative products and services are for research use only and not for therapeutic or diagnostic use.

Altogex and All other trademarks mentioned in this document are the property of their respective owners and are used for identification purposes only. Copyright © 2020 Altogex Biotech, Inc. and/or its affiliates. Altogex is a premium product manufactured by Altogex Biotech, Inc. All rights reserved.